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pin1 p53  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology pin1 p53
    Pin1 P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 9263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pin1/p53/10__1158_slash_1541___7786__mcr___25___1297-74-8-11
    Average 96 stars, based on 9263 article reviews
    pin1 p53 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings
    Article Snippet: Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions.. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS).. Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology.

    Article Title: The Mp ANT ‐Auxin Loop Modulates Marchantia polymorpha Development
    Article Snippet: Subsequently, the cell wall was degraded with 1% driselase (Sigma, D8037), 1% BSA (Sigma, A9647) at 37°C for 45 min and washed with 1× PBS 0.2% Tween 20 for 15 min. Posteriorly, the samples were permeated in 1× PBS 2% Tween 20 for 2 h, on ice. .. For labeling, samples were incubated with primary polyclonal anti‐IAA antibody (Agrisera, AS09 445) and polyclonal anti‐PIN1 (Santa Cruz Biotechnology, aP‐20: sc‐27163) at a 1:1000 dilution, and incubated overnight at 4°C. ..

    Article Title: The peptidyl-prolyl isomerase Pin1 controls GM-CSF-induced priming of NADPH oxidase in human neutrophils and priming at inflammatory sites.
    Article Snippet: .. The membranes were incubated overnight at 4 ̊C in solution containing specific relevant primary antibodies; anti-phospho-S345 (1:10000) and p47phox(1:5000) or anti-Pin1 (1/ 1000), followed by incubation with secondary antibodies (Santa Cruz, Heidelberg, Germany). .. Blots were visualized by using ECL Western blotting reagents (Amersham Pharmacia).

    Article Title: PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB.
    Article Snippet: .. The membrane was incubated with one of these primary antibodies: anti-PIN1 (G8, sc-46660, mouse monoclonal; Santa Cruz, Dallas, TX, USA), anti-HA (C29F4, rabbit monoclonal; CST, Danvers, MA, USA), anti-HA (12CA5, mouse monoclonal; Roche), anti-Flag (2368, rabbit polyclonal; CST), anti-GST (ab19256, rabbit polyclonal; Abcam, Cambridge, UK), anti-NFIB (HPA003956, rabbit polyclonal; Sigma, St. Louis, MO, USA), anti-NFIA (ARP32714; Aviva, San Diego, CA, USA), anti-NFIX (ARP32717; Aviva), anti-NFI (H300, sc-5567, rabbit polyclonal; Santa Cruz), anti-GAPDH (6C5, sc-32233, mouse monoclonal; Santa Cruz), or anti-b-Actin (C4, sc47778, mouse monoclonal; Santa Cruz). .. Next, anti-rabbit IgG-HRP (7074; CST) or anti-mouse IgG-HRP (7076; CST) were used as secondary antibodies.

    Article Title: PIN 1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB
    Article Snippet: .. The membrane was incubated with one of these primary antibodies: anti‐PIN1 (G8, sc‐46660, mouse monoclonal; Santa Cruz, Dallas, TX, USA), anti‐HA (C29F4, rabbit monoclonal; CST, Danvers, MA, USA), anti‐HA (12CA5, mouse monoclonal; Roche), anti‐Flag (2368, rabbit polyclonal; CST), anti‐GST (ab19256, rabbit polyclonal; Abcam, Cambridge, UK), anti‐NFIB (HPA003956, rabbit polyclonal; Sigma, St. Louis, MO, USA), anti‐NFIA (ARP32714; Aviva, San Diego, CA, USA), anti‐NFIX (ARP32717; Aviva), anti‐NFI (H300, sc‐5567, rabbit polyclonal; Santa Cruz), anti‐GAPDH (6C5, sc‐32233, mouse monoclonal; Santa Cruz), or anti‐β‐Actin (C4, sc47778, mouse monoclonal; Santa Cruz). .. Next, anti‐rabbit IgG‐HRP (7074; CST) or anti‐mouse IgG‐HRP (7076; CST) were used as secondary antibodies.

    Blocking Assay:

    Article Title: Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings
    Article Snippet: Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions.. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS).. Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology.

    other:

    Article Title: Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal
    Article Snippet: Anti-PIN1, mouse IgG2 (1:1000) , Santa Cruz , 46660 (1:000); RRID: AB_628132.

    Labeling:

    Article Title: The Mp ANT ‐Auxin Loop Modulates Marchantia polymorpha Development
    Article Snippet: Subsequently, the cell wall was degraded with 1% driselase (Sigma, D8037), 1% BSA (Sigma, A9647) at 37°C for 45 min and washed with 1× PBS 0.2% Tween 20 for 15 min. Posteriorly, the samples were permeated in 1× PBS 2% Tween 20 for 2 h, on ice. .. For labeling, samples were incubated with primary polyclonal anti‐IAA antibody (Agrisera, AS09 445) and polyclonal anti‐PIN1 (Santa Cruz Biotechnology, aP‐20: sc‐27163) at a 1:1000 dilution, and incubated overnight at 4°C. ..

    Membrane:

    Article Title: PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB.
    Article Snippet: .. The membrane was incubated with one of these primary antibodies: anti-PIN1 (G8, sc-46660, mouse monoclonal; Santa Cruz, Dallas, TX, USA), anti-HA (C29F4, rabbit monoclonal; CST, Danvers, MA, USA), anti-HA (12CA5, mouse monoclonal; Roche), anti-Flag (2368, rabbit polyclonal; CST), anti-GST (ab19256, rabbit polyclonal; Abcam, Cambridge, UK), anti-NFIB (HPA003956, rabbit polyclonal; Sigma, St. Louis, MO, USA), anti-NFIA (ARP32714; Aviva, San Diego, CA, USA), anti-NFIX (ARP32717; Aviva), anti-NFI (H300, sc-5567, rabbit polyclonal; Santa Cruz), anti-GAPDH (6C5, sc-32233, mouse monoclonal; Santa Cruz), or anti-b-Actin (C4, sc47778, mouse monoclonal; Santa Cruz). .. Next, anti-rabbit IgG-HRP (7074; CST) or anti-mouse IgG-HRP (7076; CST) were used as secondary antibodies.

    Article Title: PIN 1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB
    Article Snippet: .. The membrane was incubated with one of these primary antibodies: anti‐PIN1 (G8, sc‐46660, mouse monoclonal; Santa Cruz, Dallas, TX, USA), anti‐HA (C29F4, rabbit monoclonal; CST, Danvers, MA, USA), anti‐HA (12CA5, mouse monoclonal; Roche), anti‐Flag (2368, rabbit polyclonal; CST), anti‐GST (ab19256, rabbit polyclonal; Abcam, Cambridge, UK), anti‐NFIB (HPA003956, rabbit polyclonal; Sigma, St. Louis, MO, USA), anti‐NFIA (ARP32714; Aviva, San Diego, CA, USA), anti‐NFIX (ARP32717; Aviva), anti‐NFI (H300, sc‐5567, rabbit polyclonal; Santa Cruz), anti‐GAPDH (6C5, sc‐32233, mouse monoclonal; Santa Cruz), or anti‐β‐Actin (C4, sc47778, mouse monoclonal; Santa Cruz). .. Next, anti‐rabbit IgG‐HRP (7074; CST) or anti‐mouse IgG‐HRP (7076; CST) were used as secondary antibodies.

    Western Blot:

    Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth
    Article Snippet: The Anti-ERK1/2 (#4695) (1:2000 for WB), Anti-ERK1/2 pT202/pY204 (#4370) (1:1000 for WB), Anti-AKT(#4685) (1:2000 for WB), Anti-AKT pS473 (#4060) (1:1000 for WB), Anti-c-Jun (#9165) (1:2000 for WB), Anti-c-Jun pS73 (#9164) (1:1000 for WB), Anti-c-SRC pY416 (#2101) (1:1000 for WB), AntiAcetyl-CoA Carboxylase (ACC) (#3676S) (1:1000 for WB), Anti-ACC pS79 (#11818S) (1:1000 for WB), Anti-MAPK/APK2 (#3042) (1:2000 for WB), Anti-MAPKAPK-2 pT222 (#3316S) (1:1000 for WB) were purchased from Cell Signaling Technology (All antibodies from CST were diluted 1:1000 for WB). .. Anti-GST (sc-138) (1:3000 for WB), Anti-Importin !1 (sc-55538) (1:1000 for WB), Anti-c-SRC (sc-8056) (1:1000 for WB), Anti-CK2a (sc373894) (1:1000 for WB), Anti-PIN1 (sc-46660) (1:1000 for WB), Anti-TAZ (sc-518026) (1:200 for IHC and 1:1000 for WB), anti-CTGF (sc-101586) (1:100 for IHC) were obtained from Santa Cruz Biotechnology. .. Anti-"-actin (GB11001) (1:3000 for WB) were purchased from Servicebio. anti-Flag (F3165) (1:200 for IF and 1:3000 for WB), anti-HA (H9658) (1:3000 for WB) were purchased from Sigma-Aldrich.

    Immunohistochemistry:

    Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth
    Article Snippet: The Anti-ERK1/2 (#4695) (1:2000 for WB), Anti-ERK1/2 pT202/pY204 (#4370) (1:1000 for WB), Anti-AKT(#4685) (1:2000 for WB), Anti-AKT pS473 (#4060) (1:1000 for WB), Anti-c-Jun (#9165) (1:2000 for WB), Anti-c-Jun pS73 (#9164) (1:1000 for WB), Anti-c-SRC pY416 (#2101) (1:1000 for WB), AntiAcetyl-CoA Carboxylase (ACC) (#3676S) (1:1000 for WB), Anti-ACC pS79 (#11818S) (1:1000 for WB), Anti-MAPK/APK2 (#3042) (1:2000 for WB), Anti-MAPKAPK-2 pT222 (#3316S) (1:1000 for WB) were purchased from Cell Signaling Technology (All antibodies from CST were diluted 1:1000 for WB). .. Anti-GST (sc-138) (1:3000 for WB), Anti-Importin !1 (sc-55538) (1:1000 for WB), Anti-c-SRC (sc-8056) (1:1000 for WB), Anti-CK2a (sc373894) (1:1000 for WB), Anti-PIN1 (sc-46660) (1:1000 for WB), Anti-TAZ (sc-518026) (1:200 for IHC and 1:1000 for WB), anti-CTGF (sc-101586) (1:100 for IHC) were obtained from Santa Cruz Biotechnology. .. Anti-"-actin (GB11001) (1:3000 for WB) were purchased from Servicebio. anti-Flag (F3165) (1:200 for IF and 1:3000 for WB), anti-HA (H9658) (1:3000 for WB) were purchased from Sigma-Aldrich.

    Protein Concentration:

    Article Title: Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes
    Article Snippet: .. The total protein concentration was determined using the BCA Protein Assay kit (Pierce), and 30 μg of protein was separated by reducing SDS‒PAGE on 10% or 15% Bis‒Tris gels, transferred to PVDF membranes (EMD Millipore) and probed with different primary antibodies:anti-TSG101 (1:1,000 dilution, ab30871, Abcam), anti-CD9 (1:500 dilution, sc-13118, Santa Cruz, CA, USA), anti-CD63 (1:500, sc-5275, Santa Cruz, CA, USA), anti-CD81 (1:500, sc-166029, Santa Cruz, CA, USA), anti-PPARγ (1:1,000, ab17860, Abcam), anti-CEBPβ (1:1,000, ab32358, Abcam), anti-FABP4 (1:1,000, ab92501, Abcam), anti-FASN (1:1,000, ab128870, Abcam), anti-ATGL (1:1,000, sc-365278, Santa Cruz, CA, USA), anti-HSL (1:1,000, ab45422, Abcam), anti-MINK1 (1:500, NBP1-22989, Novus), anti-PIN1 (1:500, SC-46660, Santa Cruz, CA, USA), anti-SUMO1 (1:1,000, ab32058, Abcam) and anti-β-tubulin (1:1,000, sc-166729, Santa Cruz, CA, USA) at 4 °C overnight. .. Then, the cells were incubated with a goat anti-mouse IgG secondary antibody (1:1,000, 14709, CST) or a goat anti-rabbit IgG secondary antibody (1:1,000, 14708, CST) for 1 h at room temperature.

    Bicinchoninic Acid Protein Assay:

    Article Title: Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes
    Article Snippet: .. The total protein concentration was determined using the BCA Protein Assay kit (Pierce), and 30 μg of protein was separated by reducing SDS‒PAGE on 10% or 15% Bis‒Tris gels, transferred to PVDF membranes (EMD Millipore) and probed with different primary antibodies:anti-TSG101 (1:1,000 dilution, ab30871, Abcam), anti-CD9 (1:500 dilution, sc-13118, Santa Cruz, CA, USA), anti-CD63 (1:500, sc-5275, Santa Cruz, CA, USA), anti-CD81 (1:500, sc-166029, Santa Cruz, CA, USA), anti-PPARγ (1:1,000, ab17860, Abcam), anti-CEBPβ (1:1,000, ab32358, Abcam), anti-FABP4 (1:1,000, ab92501, Abcam), anti-FASN (1:1,000, ab128870, Abcam), anti-ATGL (1:1,000, sc-365278, Santa Cruz, CA, USA), anti-HSL (1:1,000, ab45422, Abcam), anti-MINK1 (1:500, NBP1-22989, Novus), anti-PIN1 (1:500, SC-46660, Santa Cruz, CA, USA), anti-SUMO1 (1:1,000, ab32058, Abcam) and anti-β-tubulin (1:1,000, sc-166729, Santa Cruz, CA, USA) at 4 °C overnight. .. Then, the cells were incubated with a goat anti-mouse IgG secondary antibody (1:1,000, 14709, CST) or a goat anti-rabbit IgG secondary antibody (1:1,000, 14708, CST) for 1 h at room temperature.



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    Image Search Results


    A. Western blot of SDS insoluble PIN1, 6 young and 6 old. B . Western blot of SDS insoluble CST3, 6 young and 6 old. C . Western blot of Sarkosyl insoluble MAPT (Tau), 4 young and 4 geriatric. D . Western blot of sarkosyl insoluble HTT, 4 young and 4 geriatric. For all blots each lane represents an independent biological sample (individual donor).

    Journal: bioRxiv

    Article Title: Shifts in protein aggregate stability define proteostasis decline in the aging human brain

    doi: 10.64898/2026.03.27.714902

    Figure Lengend Snippet: A. Western blot of SDS insoluble PIN1, 6 young and 6 old. B . Western blot of SDS insoluble CST3, 6 young and 6 old. C . Western blot of Sarkosyl insoluble MAPT (Tau), 4 young and 4 geriatric. D . Western blot of sarkosyl insoluble HTT, 4 young and 4 geriatric. For all blots each lane represents an independent biological sample (individual donor).

    Article Snippet: Membranes were blocked in 5% non-fat milk in TBS-T for 1 hour at room temperature, then incubated overnight at 4°C on a rocker with primary antibodies against PIN1 (mouse monoclonal, 1:2000, Cat. No. 68127-1-Ig, Proteintech), Cystatin C (CST3 rabbit polyclonal, 1:6000, Cat. No. 12245-1-AP, Proteintech), total tau (Tau5, mouse monoclonal, 1:500, Cat. No. AHB0042, Invitrogen), and HTT (rabbit polyclonal, N-terminal, 1:150, Cat. No. H7540, Sigma-Aldrich).

    Techniques: Western Blot