pin1 p53 (Santa Cruz Biotechnology)
Structured Review
Pin1 P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 9263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pin1/p53/10__1158_slash_1541___7786__mcr___25___1297-74-8-11
Average 96 stars, based on 9263 article reviews
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Incubation:Article Title: Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings Article Snippet: Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions.. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS).. Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology. Article Title: The Mp ANT ‐Auxin Loop Modulates Marchantia polymorpha Development Article Snippet: Subsequently, the cell wall was degraded with 1% driselase (Sigma, D8037), 1% BSA (Sigma, A9647) at 37°C for 45 min and washed with 1× PBS 0.2% Tween 20 for 15 min. Posteriorly, the samples were permeated in 1× PBS 2% Tween 20 for 2 h, on ice. .. For labeling, samples were incubated with primary polyclonal anti‐IAA antibody (Agrisera, AS09 445) and polyclonal Article Title: The peptidyl-prolyl isomerase Pin1 controls GM-CSF-induced priming of NADPH oxidase in human neutrophils and priming at inflammatory sites. Article Snippet: .. The membranes were incubated overnight at 4 ̊C in solution containing specific relevant primary antibodies; anti-phospho-S345 (1:10000) and p47phox(1:5000) or Article Title: PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB. Article Snippet: .. The membrane was incubated with one of these primary antibodies: Article Title: PIN 1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB Article Snippet: .. The membrane was incubated with one of these primary antibodies: Blocking Assay:Article Title: Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings Article Snippet: Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions.. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS).. Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology. other:Article Title: Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal Article Snippet: Anti-PIN1, Labeling:Article Title: The Mp ANT ‐Auxin Loop Modulates Marchantia polymorpha Development Article Snippet: Subsequently, the cell wall was degraded with 1% driselase (Sigma, D8037), 1% BSA (Sigma, A9647) at 37°C for 45 min and washed with 1× PBS 0.2% Tween 20 for 15 min. Posteriorly, the samples were permeated in 1× PBS 2% Tween 20 for 2 h, on ice. .. For labeling, samples were incubated with primary polyclonal anti‐IAA antibody (Agrisera, AS09 445) and polyclonal Membrane:Article Title: PIN1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB. Article Snippet: .. The membrane was incubated with one of these primary antibodies: Article Title: PIN 1 is a novel interaction partner and a negative upstream regulator of the transcription factor NFIB Article Snippet: .. The membrane was incubated with one of these primary antibodies: Western Blot:Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth Article Snippet: The Anti-ERK1/2 (#4695) (1:2000 for WB), Anti-ERK1/2 pT202/pY204 (#4370) (1:1000 for WB), Anti-AKT(#4685) (1:2000 for WB), Anti-AKT pS473 (#4060) (1:1000 for WB), Anti-c-Jun (#9165) (1:2000 for WB), Anti-c-Jun pS73 (#9164) (1:1000 for WB), Anti-c-SRC pY416 (#2101) (1:1000 for WB), AntiAcetyl-CoA Carboxylase (ACC) (#3676S) (1:1000 for WB), Anti-ACC pS79 (#11818S) (1:1000 for WB), Anti-MAPK/APK2 (#3042) (1:2000 for WB), Anti-MAPKAPK-2 pT222 (#3316S) (1:1000 for WB) were purchased from Cell Signaling Technology (All antibodies from CST were diluted 1:1000 for WB). .. Anti-GST (sc-138) (1:3000 for WB), Anti-Importin !1 (sc-55538) (1:1000 for WB), Anti-c-SRC (sc-8056) (1:1000 for WB), Anti-CK2a (sc373894) (1:1000 for WB), Immunohistochemistry:Article Title: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth Article Snippet: The Anti-ERK1/2 (#4695) (1:2000 for WB), Anti-ERK1/2 pT202/pY204 (#4370) (1:1000 for WB), Anti-AKT(#4685) (1:2000 for WB), Anti-AKT pS473 (#4060) (1:1000 for WB), Anti-c-Jun (#9165) (1:2000 for WB), Anti-c-Jun pS73 (#9164) (1:1000 for WB), Anti-c-SRC pY416 (#2101) (1:1000 for WB), AntiAcetyl-CoA Carboxylase (ACC) (#3676S) (1:1000 for WB), Anti-ACC pS79 (#11818S) (1:1000 for WB), Anti-MAPK/APK2 (#3042) (1:2000 for WB), Anti-MAPKAPK-2 pT222 (#3316S) (1:1000 for WB) were purchased from Cell Signaling Technology (All antibodies from CST were diluted 1:1000 for WB). .. Anti-GST (sc-138) (1:3000 for WB), Anti-Importin !1 (sc-55538) (1:1000 for WB), Anti-c-SRC (sc-8056) (1:1000 for WB), Anti-CK2a (sc373894) (1:1000 for WB), Protein Concentration:Article Title: Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes Article Snippet: .. The total protein concentration was determined using the BCA Protein Assay kit (Pierce), and 30 μg of protein was separated by reducing SDS‒PAGE on 10% or 15% Bis‒Tris gels, transferred to PVDF membranes (EMD Millipore) and probed with different primary antibodies:anti-TSG101 (1:1,000 dilution, ab30871, Abcam), anti-CD9 (1:500 dilution, sc-13118, Santa Cruz, CA, USA), anti-CD63 (1:500, sc-5275, Santa Cruz, CA, USA), anti-CD81 (1:500, sc-166029, Santa Cruz, CA, USA), anti-PPARγ (1:1,000, ab17860, Abcam), anti-CEBPβ (1:1,000, ab32358, Abcam), anti-FABP4 (1:1,000, ab92501, Abcam), anti-FASN (1:1,000, ab128870, Abcam), anti-ATGL (1:1,000, sc-365278, Santa Cruz, CA, USA), anti-HSL (1:1,000, ab45422, Abcam), anti-MINK1 (1:500, NBP1-22989, Novus), Bicinchoninic Acid Protein Assay:Article Title: Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes Article Snippet: .. The total protein concentration was determined using the BCA Protein Assay kit (Pierce), and 30 μg of protein was separated by reducing SDS‒PAGE on 10% or 15% Bis‒Tris gels, transferred to PVDF membranes (EMD Millipore) and probed with different primary antibodies:anti-TSG101 (1:1,000 dilution, ab30871, Abcam), anti-CD9 (1:500 dilution, sc-13118, Santa Cruz, CA, USA), anti-CD63 (1:500, sc-5275, Santa Cruz, CA, USA), anti-CD81 (1:500, sc-166029, Santa Cruz, CA, USA), anti-PPARγ (1:1,000, ab17860, Abcam), anti-CEBPβ (1:1,000, ab32358, Abcam), anti-FABP4 (1:1,000, ab92501, Abcam), anti-FASN (1:1,000, ab128870, Abcam), anti-ATGL (1:1,000, sc-365278, Santa Cruz, CA, USA), anti-HSL (1:1,000, ab45422, Abcam), anti-MINK1 (1:500, NBP1-22989, Novus), |
